Estève, Pierre-Olivier; Tremblay, Pierre; Houde, Michel; St-Pierre, Yves 
ORCID: https://orcid.org/0000-0002-1948-2041 et Mandeville, Rosemonde
(1998).
In vitro expression of MMP-2 and MMP-9 in glioma cells following exposure to inflammatory mediators
Biochimica et Biophysica Acta - Molecular Cell Research
, vol. 1403
, nº 1.
pp. 85-96.
DOI: 10.1016/S0167-4889(98)00020-2.
Résumé
Progression of glioma is associated with local degenerative processes which are attributed to the activity of gelatinases. As glioma cells are candidate for secretion of these enzymes, we have studied in vitro the potential of cytokines (interleukin-1α (IL-1), tumor necrosis factor-alpha (TNFα) and transforming growth factor-beta (TGFβ2)) to regulate the expression of gelatinase A and B (Gels A and B, respectively) in two glioma cells of human (A172) and rat origin (C6). We showed that IL-1 and TNFα both induced gene expression and protein secretion of Gel B in both cell lines, as revealed by RT-PCR and gelatin zymography, respectively. In C6 cells, TNFα had no effect on Gel A constitutive expression while IL-1 increased its production, but only at high doses. We have also demonstrated that TGFβ2 inhibited both IL-1- or TNFα-induced gene expression and Gel B production in a dose-dependent manner but had no effect on Gel A secretion. The effect of TGFβ2 on Gel B secretion was reversed by phorbol myristate acetate (PMA). Taken together, these data suggest that IL-1, TNFα and TGFβ2 tightly regulate Gel B secretion in glioma cells, an enzyme which is believed to play an important role in the local invasion of brain tissue by tumor cells.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | C6 glioma; Gelatinase; Interleukin-1; Transforming growth factor-β; Tumor necrosis factor-α |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 07 juill. 2025 18:29 |
| Dernière modification: | 07 juill. 2025 18:29 |
| URI: | https://espace.inrs.ca/id/eprint/14653 |
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