Estève, Pierre-Olivier; Robledo, Olivier; Potworowski, Edouard F. et St-Pierre, Yves 
ORCID: https://orcid.org/0000-0002-1948-2041
(2002).
Induced expression of MMP-9 in C6 glioma cells is inhibited by PDGF via a PI 3-kinase-dependent pathway
Biochemical and Biophysical Research Communications
, vol. 296
, nº 4.
pp. 864-869.
DOI: 10.1016/s0006-291x(02)02008-9.
Résumé
The involvement of phosphatidylinositol 3 (PI 3)-kinase in the signalling pathways leading to MMP-9 expression in glioma cells remains unclear. Here, we report that PI 3-kinase inhibits MMP-9 expression induced by either IL-1 or TNF-alpha in rat C6 glioma cells. Using zymography and semi-quantitative RT-PCR analysis, we showed that treatment of C6 cells with wortmannin, an inhibitor of PI 3-kinase activity, potentiated the expression of MMP-9 induced by both cytokines. In contrast, platelet-derived growth factor (PDGF), an inducer of PI 3-kinase activity in C6 cells, inhibited IL-1- or TNF-alpha-induced MMP-9 secretion. Accordingly, this inhibition by PDGF was prevented by wortmannin. Furthermore, stable C6 clones over-expressing the dominant-negative form the regulatory subunit of PI 3-kinase potentiated the expression of MMP-9 induced by IL-1 or TNF-alpha. Taken together, these results suggest that PI 3-kinase may act as a negative regulator of MMP-9 expression in C6 glioma cells.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Glioma; MMP-9; PI 3-kinase; PDGF; IL-1; TNF-α; Wortmannin |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 03 mars 2025 20:58 |
| Dernière modification: | 03 mars 2025 20:58 |
| URI: | https://espace.inrs.ca/id/eprint/14652 |
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