Ennaciri, Jamila et Girard, Denis 
ORCID: https://orcid.org/0000-0002-3342-5027
(2009).
IL-4R{alpha}, a New Member that Associates with Syk Kinase: Implication in IL-4-Induced Human Neutrophil Functions
Journal of Immunology
, vol. 183
, nº 8.
pp. 5261-5269.
DOI: 10.4049/jimmunol.0900109.
Résumé
Although Syk has been reported to be assocd. with IL-2Rα and IL-15Rα in some hematopoietic cells, its assocn. has never been investigated in the IL-4/IL-4R system. In this study, we demonstrate for the first time that Syk is constitutively assocd. with IL-4Rα in human polymorphonuclear neutrophils (PMNs) and that IL-4 stimulation increases the amt. of Syk assocd. with IL-4Rα. Moreover, upon IL-4 treatment, a pool of Syk assocd. with IL-4Rα is phosphorylated. We also report that such assocn. is not unique to PMNs because Syk assocs. with IL-4Rα in Raji and in PBMC cells. Stimulation of PMNs by IL-4 increased the amt. of Syk assocd. with PLC-γ2, pAkt, and α-tubulin. Pretreatment of cells with the Syk-selective inhibitor piceatannol or Syk inhibitor II, significantly inhibited the ability of IL-4 to enhance phagocytosis and cell adhesion and to delay apoptosis, and these results correlate with the ability of piceatannol to reduce Syk activation and its assocn. with IL-4Rα. Down-regulation of Syk by antisense techniques demonstrates the importance of Syk in the antiapoptotic effect of IL-4. We conclude that assocn. of Syk to IL-4Rα is of biol. significance and that IL-4Rα is a new candidate to be added to the few cytokine receptor components which assoc. with Syk.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 30 juin 2024 17:59 |
| Dernière modification: | 30 juin 2024 17:59 |
| URI: | https://espace.inrs.ca/id/eprint/14648 |
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