Can enzyme engineering benefit from the modulation of protein motions? Lessons learned from NMR relaxation dispersion experiments

Doucet, Nicolas ORCID: https://orcid.org/0000-0002-1952-9380 (2011). Can enzyme engineering benefit from the modulation of protein motions? Lessons learned from NMR relaxation dispersion experiments Protein and Peptide Letters , vol. 18 , nº 4. pp. 336-343. DOI: 10.2174/092986611794653950.

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Résumé

Despite impressive progress in protein engineering and design, our ability to create new and efficient enzyme activities remains a laborious and time-consuming endeavor. In the past few years, intricate combinations of rational mutagenesis, directed evolution and computational methods have paved the way to exciting engineering examples and are now offering a new perspective on the structural requirements of enzyme activity. However, these structure-function analyses are usually guided by the time-averaged static models offered by enzyme crystal structures, which often fail to describe the functionally relevant 'invisible states' adopted by proteins in space and time. To alleviate such limitations, NMR relaxation dispersion expts. coupled to mutagenesis studies have recently been applied to the study of enzyme catalysis, effectively complementing 'structure-function' analyses with 'flexibility-function' investigation. In addn. to offering quant., site-specific information to help characterize residue motion, these NMR methods are now being applied to enzyme engineering purposes, providing a powerful tool to help characterize the effects of controlling long-range networks of flexible residues affecting enzyme function. Recent advancements in this emerging field are presented here, with particular attention to mutagenesis reports highlighting the relevance of NMR relaxation dispersion tools in enzyme engineering.

Type de document:	Article
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	24 mars 2024 21:39
Dernière modification:	24 mars 2024 21:39
URI:	https://espace.inrs.ca/id/eprint/14608

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