Denis, Jérôme; Majeau, Nathalie; Acosta-Ramirez, Elizabeth; Savard, Christian; Bedard, Marie-Claude; Simard, Sabrina; Lecours, Katia; Bolduc, Marilène; Pare, Christine; Willems, Bernard; Shoukry, Naglaa; Tessier, Philippe; Lacasse, Patrick; Lamarre, Alain 
ORCID: https://orcid.org/0000-0002-7913-871X; Lapointe, Réjean; Lopez Macias, Constantino et Leclerc, Denis
(2007).
Immunogenicity of papaya mosaic virus-like particles fused to a hepatitis C virus epitope: Evidence for the critical function of multimerization
Virology
, vol. 363
, nº 1.
pp. 59-68.
DOI: 10.1016/j.virol.2007.01.011.
Résumé
Plant-virus-based vaccines have emerged as a promising avenue in vaccine development. This report describes the engineering of an innovative vaccine platform using the papaya mosaic virus (PapMV) capsid protein (CP) as a carrier protein and a C-terminal fused hepatitis C virus (HCV) E2 epitope as the immunogenic target. Two antigen organizations of the PapMV-based vaccines were tested: a virus-like-particle (VLP; PapMVCP-E2) and a monomeric form (PapMVCP(27-215)-E2). While the two forms of the vaccine were both shown to be actively internalized in vitro in bone-marrow-derived antigen presenting cells (APCs), immunogenicity was demonstrated to be strongly dependent on antigen organization. Indeed, C3H/HeJ mice injected twice with the multimeric VLP vaccine showed a long-lasting humoral response (more than 120 days) against both the CP and the fused HCV E2 epitope. The antibody profile (production of IgG1, IgG2a, IgG2b, IgG3) suggests a Th1/Th2 response. Immunogenicity of the PapMV vaccine platform was not observed when the monomer PapMVCP-E2 was injected. These results demonstrate for the first time the potential of the PapMV vaccine platform and the critical function of multimerization in its immunogenicity.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Vaccination; Virus-like particles (VLPs); Chimeric plant virus; Epitope carrier |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 31 oct. 2024 01:45 |
| Dernière modification: | 31 oct. 2024 01:45 |
| URI: | https://espace.inrs.ca/id/eprint/14581 |
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