Couillard, Julie; Demers, Mélanie; Lavoie, Geneviève et St-Pierre, Yves 
ORCID: https://orcid.org/0000-0002-1948-2041
(2006).
The role of DNA hypomethylation in the control of stromelysin gene expression
Biochemical and Biophysical Research Communications
, vol. 342
, nº 4.
pp. 1233-1239.
DOI: 10.1016/j.bbrc.2006.02.068.
Résumé
Genome-wide DNA hypomethylation is a critical mechanism Underlying neoplastic transformation. Thus, genes that are suppressed in normal tissues or in specific cell types may become aberrantly expressed in neoplasia. To determine whether DNA methylation call modulate matrix metalloproteinase (mmp) gene expression, we have used a genetically engineered cell line in which both key DNA methyltransferase genes, Dnmt-1 and Dnmt-3b, were removed by homologous recombination. We found that cells bearing a dual knock-out Of both Dnmt-1 and Dnmt-3b genes induced de novo expression of mmp-3 gene, but not that of mmp-1 and mmp-2. Furthermore, treatment of the wild-type cells with DNA methylase inhibitors 5-aza-dC and zebularine also induced mmp-3 gene expression. On the other hand, in vitro methylation of the mmp-3 promoter suppressed its transcription activity. Finally, we found that induction of mmp-3 and mmp-10 gene expression by hypomethylation was cell-specific, suggesting that epigenetic changes may predispose cells to express stromelysin genes. (c) 2006 Elsevier Inc. All rights reserved.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Matrix metalloproteinase; DNA methylation; Colon carcinoma; Lymphoma; Transcription; 5-Aza-2′-deoxycytidine; Zebularine; Gene transcription; Cancer biology |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 22 oct. 2024 19:54 |
| Dernière modification: | 22 oct. 2024 19:54 |
| URI: | https://espace.inrs.ca/id/eprint/14518 |
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