Bouchard, Amélie; Ratthé, Claude et Girard, Denis 
ORCID: https://orcid.org/0000-0002-3342-5027
(2004).
Interleukin-15 delays human neutrophil apoptosis by intracellular events and not via extracellular factors: Role of Mcl-1 and decreased activity of caspase-3 and caspase-8
Journal of Leukocyte Biology
, vol. 75
, nº 5.
pp. 893-900.
DOI: 10.1189/jlb.1103585.
Résumé
Interleukin-15 (IL-15) induces the de novo protein synthesis of intracellular polypeptides and delays neutrophil apoptosis by a mechanism that is still unclear. Herein, we investigated the potential antiapoptotic role of newly synthesized proteins released into the external milieu in IL-15-induced neutrophils. We found that IL-15 induces the de novo synthesis of an approximately 23-kDa protein, representing the predominant protein detected in the milieu, and identified it as IL-1 receptor antagonist (IL-1Ra) by Western blot and immunoprecipitation. We quantified IL-1Ra, IL-1alpha, and IL-1beta concentrations by enzyme-linked immunosorbent assay in intracellular and extracellular fractions from IL-15-induced neutrophils and found that IL-15 does not increase IL-1alpha or IL-1beta production but induces IL-1Ra release. Also, we demonstrated that IL-1Ra does not modulate apoptosis, even at a concentration 250 times greater than that measured in the external milieu. In contrast to granulocyte macrophage-colony stimulating factor, the supernatant harvested from IL-15-induced neutrophils was devoid of antiapoptotic activity. Addition of cycloheximide demonstrates that IL-15 delays apoptosis via de novo synthesis of intracellular proteins and that it increases myeloid cell differentiation factor-1 stability. We demonstrated also that IL-15 decreases the activity of caspase-3 and caspase-8, resulting in an inhibition of vimentin cleavage. Our results indicate that IL-15 can activate an anti-inflammatory loop, based on its ability to induce the synthesis of IL-1Ra by neutrophils. We conclude that IL-15 delays human neutrophil apoptosis by intracellular events and not via extracellular factors.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | cytokine; inflammation; de novo protein synthesis |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 15 nov. 2024 20:20 |
| Dernière modification: | 15 nov. 2024 20:20 |
| URI: | https://espace.inrs.ca/id/eprint/14429 |
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