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Intermediate chains of exopolysaccharides from Lactobacillus rhamnosus RW-9595M increase IL-10 production by macrophages

Bleau, Christian; Monges, Alexia; Rashidan, Kianoush; Laverdure, J-P.; Lacroix, Monique ORCID logoORCID: https://orcid.org/0000-0002-2042-4033; Van Calsteren, Marie-Rose; Millette, Mathieu; Savard, Roland et Lamontagne, Lucie (2009). Intermediate chains of exopolysaccharides from Lactobacillus rhamnosus RW-9595M increase IL-10 production by macrophages Journal of Applied Microbiology , vol. 108 , nº 2. pp. 666-675. DOI: 10.1111/j.1365-2672.2009.04450.x.

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Résumé


Aims: To evaluate the immunosuppressive properties of the exopolysaccharide (EPS) from high-EPS producer Lactobacillus rhamnosus RW-9595M on inflammatory cytokines produced by macrophages. Methods and Results: The conditioned media (CM) were produced by macrophages treated with parental Lact. rhamnosus ATCC 9595 and its isogenic variant, the high-EPS producer Lact. rhamnosus RW-9595M, and the levels of TNF-alpha, IL-6, IL-10 and IL-12 were evaluated. Results revealed that CM from parental Lact. rhamnosus induced higher levels of TNF-alpha, IL-6 and IL-12 but inhibited IL-10 production, whereas its mucous variant induced low or no TNF-alpha and IL-6. Addition of purified EPS to macrophages treated with parental Lact. rhamnosus decreased the inflammatory cytokines and inhibited the metabolic activity of lymphocytes. The intermediate polysaccharide chains (16-30 units) produced by time-controlled hydrolysis of EPS increased the IL-10 produced by macrophages. Conclusions: Polysaccharide chains of EPS induced immunosuppression by the production of macrophagic anti-inflammatory IL-10. Significance and impact of the Study: These results indicate that the EPS from Lact. rhamnosus RW-9595M may be useful as a new immunosuppressive product in dairy food.

Type de document: Article
Mots-clés libres: cytokines, exopolysaccharides, Lactobacillus, lymphocytes, macrophages
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 29 juin 2024 20:56
Dernière modification: 29 juin 2024 20:56
URI: https://espace.inrs.ca/id/eprint/14424

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