Bleau, Christian; Monges, Alexia; Rashidan, Kianoush; Laverdure, J-P.; Lacroix, Monique 
ORCID: https://orcid.org/0000-0002-2042-4033; Van Calsteren, Marie-Rose; Millette, Mathieu; Savard, Roland et Lamontagne, Lucie
(2009).
Intermediate chains of exopolysaccharides from Lactobacillus rhamnosus RW-9595M increase IL-10 production by macrophages
Journal of Applied Microbiology
, vol. 108
, nº 2.
pp. 666-675.
DOI: 10.1111/j.1365-2672.2009.04450.x.
Résumé
Aims: To evaluate the immunosuppressive properties of the exopolysaccharide (EPS) from high-EPS producer Lactobacillus rhamnosus RW-9595M on inflammatory cytokines produced by macrophages. Methods and Results: The conditioned media (CM) were produced by macrophages treated with parental Lact. rhamnosus ATCC 9595 and its isogenic variant, the high-EPS producer Lact. rhamnosus RW-9595M, and the levels of TNF-alpha, IL-6, IL-10 and IL-12 were evaluated. Results revealed that CM from parental Lact. rhamnosus induced higher levels of TNF-alpha, IL-6 and IL-12 but inhibited IL-10 production, whereas its mucous variant induced low or no TNF-alpha and IL-6. Addition of purified EPS to macrophages treated with parental Lact. rhamnosus decreased the inflammatory cytokines and inhibited the metabolic activity of lymphocytes. The intermediate polysaccharide chains (16-30 units) produced by time-controlled hydrolysis of EPS increased the IL-10 produced by macrophages. Conclusions: Polysaccharide chains of EPS induced immunosuppression by the production of macrophagic anti-inflammatory IL-10. Significance and impact of the Study: These results indicate that the EPS from Lact. rhamnosus RW-9595M may be useful as a new immunosuppressive product in dairy food.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | cytokines, exopolysaccharides, Lactobacillus, lymphocytes, macrophages |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 29 juin 2024 20:56 |
| Dernière modification: | 29 juin 2024 20:56 |
| URI: | https://espace.inrs.ca/id/eprint/14424 |
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