Quantitative analysis of the relative transcript levels of four chlorophenol reductive Dehalogenase genes in Desulfitobacterium hafniense PCP-1 exposed to chlorophenols

Bisaillon, Ariane; Beaudet, Réjean; Lépine, François et Villemur, Richard ORCID: https://orcid.org/0000-0001-9768-8937 (2011). Quantitative analysis of the relative transcript levels of four chlorophenol reductive Dehalogenase genes in Desulfitobacterium hafniense PCP-1 exposed to chlorophenols Applied and Environmental Microbiology , vol. 77 , nº 17. pp. 6261-6264. DOI: 10.1128/AEM.00390-11.

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Résumé

Relative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes (cprA2, cprA3, cprA4, and cprA5) in Desulfitobacterium hafniense PCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures, cprA2 and cprA3 were upregulated, as was cprA5, but concomitantly with the appearance of 2,4-dichlorophenol (DCP). In 3,5-DCP-amended cultures, only cprA5 was upregulated. In pentachlorophenol-amended cultures grown for 12 h, cprA2 and cprA3 were upregulated but not cprA5. cprA4 was not upregulated significantly in cultures containing any tested chlorophenols.

Type de document:	Article
Mots-clés libres:	-
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	09 mars 2024 23:04
Dernière modification:	09 mars 2024 23:04
URI:	https://espace.inrs.ca/id/eprint/14423

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