Bélizaire, Anna-Karine; Tchistiakova, Lioudmila; St-Pierre, Yves 
ORCID: https://orcid.org/0000-0002-1948-2041 et Alakhov, Valery
(2003).
Identification of a murine ICAM-1-specific peptide by subtractive phage library selection on cells
Biochemical and Biophysical Research Communications
, vol. 309
, nº 3.
pp. 625-630.
DOI: 10.1016/j.bbrc.2003.08.050.
Résumé
The ICAM-1 adhesion molecule is expressed selectively at low levels on endothelial cells but is strongly upregulated in dysfunctional endothelial cells associated with inflammation, cancer, and atherogenesis. Using COS-7 cells transfected with murine ICAM-1 (mICAM-1) as a target receptor, a phage display library was screened. Clones were selected by elution with a mAb specific for a functional epitope of ICAM-1 and a novel peptide sequence binding to the extracellular domain of mICAM-1 was identified that can potentially be used as a targeting vector aimed at dysfunctional endothelium. We further showed that the targeting specificity of the peptide was retained following its incorporation at the N terminal end of a large chimeric protein. Moreover, this chimeric protein containing the mICAM-1-specific sequence was found to inhibit ICAM-1-mediated intercellular adhesion during antigen presentation. Taken together, these results demonstrate the potential for improving the cell-selectivity and properties of therapeutical agents toward targeting adhesion molecules involved in cell-cell interactions.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | ICAM-1; Peptide; Phage display; Antigen presentation |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 25 déc. 2024 16:41 |
| Dernière modification: | 25 déc. 2024 16:41 |
| URI: | https://espace.inrs.ca/id/eprint/14398 |
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