Ayari, Samia; Dussault, Dominic; Millette, Mathieu; Hamdi, Moktar et Lacroix, Monique 
ORCID: https://orcid.org/0000-0002-2042-4033
(2010).
Response of Bacillus cereus to gamma-Irradiation in Combination with Carvacrol or Mild Heat Treatment
Journal of Agricultural and Food Chemistry
, vol. 58
, nº 14.
pp. 8217-8224.
DOI: 10.1021/jf101044f.
Résumé
Carvacrol and mild heat treatment were tested for their efficiency to increase the radiosensitivity of Bacillus cereus in broth. The bacterium was treated with gamma-irradiation alone or in combination with carvacrol at its minimal inhibitory concentration or mild heat treatment for 10 min at 45 degrees C. The effects of this combination of treatments were studied on various parameters: the bacterial viability, the modifications of the cell morphology with scanning electron microscopy (SEM), the cellular fatty acids composition of the membrane quantified by gas chromatography, the intracellular and extracellular adenosine 5'-triphosphate (ATP) concentrations, and the DNA degradation. Combined treatments resulted in additive or synergistic effects as compared to gamma-irradiation alone. A significant modification (P <= 0.05) of the fatty acid composition and unsaturation ratios was observed. Pretreatment with mild heat or carvacrol before irradiation disturbed the membrane integrity of B. cereus and induced a significant decrease (P <= 0.05) of the intracellular ATP concentration. SEM observations revealed that the cell membrane was more severely affected with combined treatment than irradiation alone. The electrophoresis analysis showed that DNA degradation by combined treatments was greater than the gamma-irradiation alone.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 28 juin 2024 19:28 |
| Dernière modification: | 28 juin 2024 19:28 |
| URI: | https://espace.inrs.ca/id/eprint/14367 |
Gestion Actions (Identification requise)
 |
Modifier la notice |