Axelsson, Fredrika; Persson, Jonas; Moreau, Emmanuel; Côté, Marie-Hélène; Lamarre, Alain 
ORCID: https://orcid.org/0000-0002-7913-871X et Ohlin, Mats
(2009).
Novel antibody specificities targeting glycoprotein B of cytomegalovirus identified by molecular library technology
New Biotechnology
, vol. 25
, nº 6.
pp. 429-436.
DOI: 10.1016/j.nbt.2009.05.003.
Résumé
Antibodies provide some protection against cytomegalovirus-mediated disease. All aspects of antibody recognition of important viral antigens are, however, not fully appreciated. Glycoprotein B (gB), a key protein in the viral membrane, participates in viral infection and it is a component of prototype vaccines. By using combinatorial antibody library and selection technology, novel antibody specificities targeting gB have now been isolated. We define a monoclonal antibody fragment able to recognize site I of antigenic domain (AD) 2, a poorly immunogenic epitope targeted by potent virus-neutralizing antibodies, in a way that is different from the binding of antibodies induced by infection but similar to those induced by vaccination. We also describe the existence of a novel epitope overlapping site I of AD-2 and AD-1, the immunodominant epitope of gB. These specificities, derived by molecular engineering, will be useful for the future assessment of humoral immune responses against this opportunistic viral infection. © 2009 Elsevier B.V. All rights reserved.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 28 juin 2024 19:26 |
| Dernière modification: | 28 juin 2024 19:26 |
| URI: | https://espace.inrs.ca/id/eprint/14365 |
Gestion Actions (Identification requise)
 |
Modifier la notice |