Aubé, Caroline; Bélanger, Simon D. et St-Pierre, Yves 
ORCID: https://orcid.org/0000-0002-1948-2041
(2010).
Lymphoma cells contribute to the augmentation of plasma sL-selectins in the serum of lymphoma-bearing mice
Leukemia and Lymphoma
, vol. 51
, nº 1.
pp. 125-31.
DOI: 10.3109/10428190903421177.
Résumé
Like many integral membrane glycoproteins, the extracellular domain of L-selectin undergoes rapid shedding, which occurs on both resting and activated host leucocytes. Incubating normal or transformed leukocytes with phorbol esters can also artificially induce shedding of L-selectin, providing multiple possibilities for the source of soluble forms of L-selectin found in the serum of patients with hematological malignancies. Here, using genetically engineered L-selectin-deficient mouse models, we have measured the release of soluble circulating forms of L-selectin in the serum of lymphoma-bearing mice. We found that L-selectin-deficient lymphoma cells could not induce an elevation of circulating soluble forms of L-selectin in normal mice, as compared to lymphoma cells expressing L-selectin. Moreover, soluble forms of L-selectin were detected in the serum in mice bearing lymphoma induced by injection of T lymphoma cells expressing L-selectins. Interestingly, we also found that lymphoma cells that are unable to shed L-selectin in vitro following exposure to phorbol ester can generate soluble forms of serum L-selectin in vivo. Taken together, these results indicate that lymphoma cells are the major contributors to levels of soluble forms of L-selectins in lymphoma-bearing mice.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 28 juin 2024 19:20 |
| Dernière modification: | 28 juin 2024 19:21 |
| URI: | https://espace.inrs.ca/id/eprint/14361 |
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