Assemand, Emma; Lacroix, Monique 
ORCID: https://orcid.org/0000-0002-2042-4033 et Mateescu, Mircea Alexandru
(2004).
Protective role of L-tyrosine in the sterilization of Ceruloplasmin therapeutic protein by gamma-irradiation
Radiation Physics and Chemistry
, vol. 71
, nº 1-2.
pp. 403-407.
DOI: 10.1016/j.radphyschem.2004.05.035.
Résumé
Gamma-irradiation of proteins in aqueous media at doses higher than 5kGy, is known to induce their aggregation (without oxygen) or degradation (in presence of oxygen). The effect of L-tyrosine (Tyr) addition before irradiation of therapeutic proteins, i.e. ceruloplasmin (CP), was examined. It was found that the presence of L-tyrosine during irradiation in oxygen free conditions prevents the protein aggregation even at doses as high as 10kGy, probably by scavenging oxygen radicals produced by irradiation. The protective role of L-tyrosine allowing the gamma-irradiation treatment of therapeutic proteins in solution, without conformational alteration was asserted by Sodium Dodecyl Sulphate - Polyacrylamide Gel Electrophoresis and by High Performance Size Exclusion Chromatography. Total viable microorganisms content evaluated by Plate Count Agar incubation for 48h at 30°C, showed that CP protein preparations gamma-irradiated in presence and absence of L-tyrosine remained sterile and maintained its enzyme activity during at least eight weeks of storage at 4°C. At day zero of irradiation, the sterility was reached in all variants (CP, CP+Tyr, Tyr) at doses higher than 4kGy. However, during storage, sterility was also found for 3kGy irradiated CP and for 2kGy irradiated CP+Tyr.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Ceruloplasmin; Tyrosine; Gamma-irradiation; Sterilization |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 15 nov. 2024 20:17 |
| Dernière modification: | 15 nov. 2024 20:17 |
| URI: | https://espace.inrs.ca/id/eprint/14360 |
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