Assemand, Emma; Lacroix, Monique 
ORCID: https://orcid.org/0000-0002-2042-4033 et Mateescu, Mircea Alexandru
(2003).
L-Tyrosine prevents aggregation of therapeutic proteins by gamma-irradiation
Biotechnology and Applied Biochemistry
, vol. 38
, nº 2.
pp. 151-156.
DOI: 10.1042/ba20030038.
Résumé
The present paper describes a protective role of L-tyrosine against aggregation of caeruloplasmin and haemoglobin therapeutic proteins during their sterilization by gamma-irradiation in the aqueous phase. Irradiation of proteins, known to induce their degradation in the presence of oxygen, generates aggregation under oxygen-free conditions. It was found that L-tyrosine present during irradiation in deoxygenated media prevents protein aggregation even at high doses (10 kGy), as asserted by SDS/PAGE and high-performance size-exclusion chromatography. The protective role Of L-tyrosine, allowing the gamma-irradiation treatment of therapeutic proteins in solution without conformational alteration, is probably exerted by scavenging oxygen radicals produced by irradiation-induced water radiolysis. It was also found that haemoglobin had a greater stability than caeruloplasmin under gamma-irradiation treatment.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | caeruloplasmin; conformational alteration; haemoglobin; protein processing; tyrosine |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 25 déc. 2024 17:13 |
| Dernière modification: | 25 déc. 2024 17:13 |
| URI: | https://espace.inrs.ca/id/eprint/14359 |
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