Arseneault, Madeleine et Vaillancourt, Cathy 
ORCID: https://orcid.org/0000-0003-0543-6244
(2005).
Characterization of the 5-HT2A serotoninergic receptor in human placental choriocarcinoma cells lines
In: 11th IFPA Meeting, 3-7 September 2005, Glasgow, England.
Résumé
We have recently demonstrated a mitogenic effect of serotonin via stimulation of 5-HT2A
receptors in placental choriocarcinoma cell lines, JEG-3 and BeWo. The mechanism
through which the 5-HT2A receptors perform this stimulating effect on placental cell proliferation is still unknown.
Objectives and methods: In the present study, we investigated, by co-immunoprecipitation and western blot analysis, the implication of 1) the Gq/11-PLC-β-MAP kinase and 2)
the JAK/STAT signaling pathways following the activation of placental 5-HT2A receptors.
Results: Our results showed that stimulation of the choriocarcinoma cell lines, JEG3 and BeWo, by a specifi c 5-HT2A receptor agonist, DOI, leads to a JAK2 and 5-HT2A
interaction as well as to a increase of the interaction between Gq/11 and 5-HT2A receptor.
Moreover, our data demonstrated that the activation of the 5-HT2A receptor in these cell
lines activates both the MEK-ERK1/2 and JAK2-STAT3 signaling pathways.
Conclusion: These results shown that the activation of the 5-HT2A receptors stimulate the
JAK2/STAT3 pathway and Erkl/2 pathway in the JEG-3 and BeWo cell lines. More studies are necessary to fi nd out which pathway or pathways are responsible for the increase
of cell proliferation by 5-HT.
| Type de document: | Document issu d'une conférence ou d'un atelier |
|---|---|
| Informations complémentaires: | P15.16 Placenta 26(8-9): A67, 2005 European Placenta Group |
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 30 oct. 2024 01:05 |
| Dernière modification: | 30 oct. 2024 01:05 |
| URI: | https://espace.inrs.ca/id/eprint/14356 |
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