Aoudjit, Fawzi; Potworowski, Edouard F. et St-Pierre, Yves 
ORCID: https://orcid.org/0000-0002-1948-2041
(1998).
Bi-Directional Induction of Matrix Metalloproteinase-9 and Tissue Inhibitor of Matrix Metalloproteinase-1 During T Lymphoma Endothelial Cell Contact: Implication of Icam-1
Journal of Immunology
, vol. 160
, nº 6.
pp. 2967-2973.
DOI: 10.4049/jimmunol.160.6.2967.
Résumé
The mechanisms that lead to the expression of matrix metalloproteinases (MMP) and tissue inhibitors of MMP (TIMPs) during the invasive process of normal and transformed T cells remain largely unknown. Since vascular cells form a dynamic tissue capable of responding to local stimuli and activating cells through the expression of cytokine receptors and specific cell adhesion molecules, we hypothesized that the firm adhesion of T lymphoma cells to endothelial cells is a critical event in the local production of MMP and TIMP. In the present work, we show that adhesion of lymphoma cells to endothelial cells induced a transient and reciprocal de novo expression of MMP-9 mRNA and enzymatic activity by both cell types, Up-regulation of MMP-9 in T lymphoma cells was concomitant to that of TIMP-1, and required direct contact with endothelial cells, Induction of MMP-9, but not of TIMP-1, was blocked by anti-LFA-1 and anti-intercellular adhesion molecule-1 Abs, indicating that induction of MMP-9 and TIMP-1 in lymphoma cells required direct, yet distinct, intercellular contact, In contrast, the induction of MMP-9 in endothelial cells by T lymphoma cells did not necessitate direct contact and could be achieved by exposure to IL-1 and TNF, or to the supernatant of T lymphoma cell culture, Together, these results demonstrate that firm adhesion of T lymphoma cells to endothelial cells participates in the production of MMP-9 in both cell types through bi- directional signaling pathways, and identify intercellular adhesion molecule-1/LFA-1 as a key interaction in the up- regulation of MMP-9 in T lymphoma cells.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 07 juill. 2025 18:20 |
| Dernière modification: | 07 juill. 2025 18:20 |
| URI: | https://espace.inrs.ca/id/eprint/14351 |
Gestion Actions (Identification requise)
 |
Modifier la notice |