Angers, Isabelle; Sancho-Shimizu, Vanessa; Descoteaux, Albert 
ORCID: https://orcid.org/0000-0002-0633-5309; Gewirtz, Andrew T et Malo, Danielle
(2006).
Tlr5 is not primarily associated with susceptibility to Salmonella Typhimurium infection in MOLF/Ei mice
Mammalian Genome
, vol. 17
, nº 5.
pp. 385-397.
DOI: 10.1007/s00335-005-0132-x.
Résumé
The extreme susceptibility to infection with Salmonella Typhimurium of wild-derived MOLF/Ei mice has been linked to one genomic region on Chromosome 1 (Ity3). A member of the Toll-like receptors family, Tlr5, located on distal Chromosome 1, was previously shown to be a candidate gene for Ity3 based on expression studies and sequencing analysis. The candidacy of Tlr5 as a Salmonella-susceptibility gene was evaluated functionally by comparing Tlr5 C57BL/6J and MOLF/Ei alleles in vitro and in vivo. In vitro studies showed that the MOLF/Ei Tlr5 allele is more transcriptionally active when the gene is removed from its natural genomic environment. This observation was supported by in vivo studies in B6.MOLF-Ity3 congenic mice that showed that mice homozygous for the MOLF/Ei allele at Ity3, including Tlr5, had an increased response to flagellin as measured by IL-6 and CXCL-1 secretion in the serum compared with parental MOLF/Ei mice. Despite the fact that both MOLF/Ei and B6.MOLF-Ity/Ity3 mice are more susceptible to Salmonella Typhimurium infection than B6.MOLF-Ity mice, they exhibit a different phenotype with respect to Tlr5 expression and Tlr5 signaling, supporting the prediction that Tlr5 is not primarily involved in the disease phenotype underlying the Ity3 locus in MOLF/Ei mice.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 22 oct. 2024 13:09 |
| Dernière modification: | 22 oct. 2024 13:09 |
| URI: | https://espace.inrs.ca/id/eprint/14346 |
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