Beauchamp, Stéphane et Lacroix, Monique 
ORCID: https://orcid.org/0000-0002-2042-4033
(2012).
Resistance of the genome of Escherichia coli and Listeria monocytogenes to irradiation evaluated by the induction of cyclobutane pyrimidine dimers and 6-4 photoproducts using gamma and UV-C radiations
Radiation Physics and Chemistry
, vol. 81
, nº 8.
pp. 1193-1197.
DOI: 10.1016/j.radphyschem.2011.11.007.
Résumé
The effect of gamma and UV-C irradn. on the prodn. of cyclobutane pyrimidine dimers (CPDs) and 6-4 photoproducts (6-4 PPs) in DNA was investigated to compare the natural resistance of the genome of a Gram-pos. bacterium and a Gram-neg. bacterium against irradn. Soln. of pure DNA and bacterial strains Listeria monocytogenes and Escherichia coli were irradiated using gamma and UV-C rays. Extd. DNA from bacteria and pure DNA samples were then analyzed by ELISA using anti-CPDs and anti-6-4 PPs monoclonal antibodies. The results show that gamma rays, as well as UV-C rays, induce the formation of CPDs and 6-4 PPs in DNA. During UV-C irradn., the three samples showed a difference in their sensitivity against formation of CPDs (P≤0.05). Pure DNA was the most sensitive while the genome of L. monocytogenes was the most resistant. Also during UV-C irradn., the genome of L. monocytogenes was the only one to show a significant resistance against formation of 6-4 PPs (P≤0.05). During gamma irradn., for both types of lesion, pure DNA and the genome of E. coli did not show significant difference in their sensitivity (P>0.05) while the genome of L. monocytogenes showed a resistance against formation of CPDs and 6-4 PPs.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Gamma irradiation; UV-C irradiation; Cyclobutane pyrimidine dimers 6-4 photoproducts |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 06 mars 2024 15:33 |
| Dernière modification: | 06 mars 2024 15:33 |
| URI: | https://espace.inrs.ca/id/eprint/13984 |
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