Characterization and description of Gabonibacter chumensis sp. nov., isolated from feces of a patient with non-small cell lung cancer treated with immunotherapy

Diop, Khoudia; Pidgeon, Reilly; Diop, Awa; Benlaïfaoui, Myriam; Belkaid, Wiam; Malo, Julie; Bernet, Eve; Veyrier, Frédéric ORCID: https://orcid.org/0000-0002-8574-0547; Jacq, Maxime; Brun, Yves; Elkrief, Arielle; Castagner, Bastien; Routy, Bertrand et Richard, Corentin (2023). Characterization and description of Gabonibacter chumensis sp. nov., isolated from feces of a patient with non-small cell lung cancer treated with immunotherapy Archives of microbiology , vol. 205 , nº 10:338. pp. 1-10. DOI: 10.1007/s00203-023-03671-0.

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Résumé

A polyphasic taxonomic approach, incorporating analysis of phenotypic features, cellular fatty acid profiles, 16S rRNA gene sequences, and determination of average nucleotide identity (ANI) plus digital DNA-DNA hybridization (dDDH), was applied to characterize an anaerobic bacterial strain designated KD22(T) isolated from human feces. 16S rRNA gene-based phylogenetic analysis showed that strain KD22(T) was found to be most closely related to species of the genus Gabonibacter. At the 16S rRNA gene level, the closest species from the strain KD22(T) corresponded with Gabonibacter massiliensis GM7(T), with a similarity of 97.58%. Cells of strain KD22(T) were Gram-negative coccobacillus, positive for indole and negative for catalase, nitrate reduction, oxidase, and urease activities. The fatty acid analysis demonstrated the presence of a high concentration of iso-C(15: 0) (51.65%). Next, the complete whole-genome sequence of strain KD22(T) was 3,368,578 bp long with 42 mol% of DNA G + C contents. The DDH and ANI values between KD22(T) and type strains of phylogenetically related species were 67.40% and 95.43%, respectively. These phylogenetic, phenotypic, and genomic results supported the affiliation of strain KD22(T) as a novel bacterial species within the genus Gabonibacter. The proposed name is Gabonibacter chumensis and the type strain is KD22(T) (= CSUR Q8104(T) = DSM 115208( T)).

Type de document:	Article
Mots-clés libres:	Bacteria; Cancer; Gabonibacter chumensis; Gut microbiota; Immunotherapy; Non-small cell lung cancer.
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	30 déc. 2023 22:44
Dernière modification:	30 déc. 2023 22:44
URI:	https://espace.inrs.ca/id/eprint/13635

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