Interaction Mechanism Between the HSV-1 Glycoprotein B and the Antimicrobial Peptide Amyloid-beta

Bourgade, Karine; Frost, Eric H.; Dupuis, Gilles; Witkowski, Jacek M.; Laurent, Benoit; Calmettes, Charles; Ramassamy, Charles ORCID: https://orcid.org/0000-0002-3252-5878; Desroches, Mathieu; Rodrigues, Serafim et Fulop, Tamas (2022). Interaction Mechanism Between the HSV-1 Glycoprotein B and the Antimicrobial Peptide Amyloid-beta Journal of Alzheimers Disease Reports , vol. 6 , nº 1. pp. 599-606. DOI: 10.3233/adr-220061.

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Résumé

Background: Unravelling the mystery of Alzheimer's disease (AD) requires urgent resolution given the worldwide increase of the aging population. There is a growing concern that the current leading AD hypothesis, the amyloid cascade hypothesis, does not stand up to validation with respect to emerging new data. Indeed, several paradoxes are being discussed in the literature, for instance, both the deposition of the amyloid-beta peptide (A beta) and the intracellular neurofibrillary tangles could occur within the brain without any cognitive pathology. Thus, these paradoxes suggest that something more fundamental is at play in the onset of the disease and other key and related pathomechanisms must be investigated. Objective: The present study follows our previous investigations on the infectious hypothesis, which posits that some pathogens are linked to late onset AD. Our studies also build upon the finding that A beta is a powerful antimicrobial agent, produced by neurons in response to viral infection, capable of inhibiting pathogens as observed in in vitro experiments. Herein, we ask what are the molecular mechanisms in play when A beta neutralizes infectious pathogens? Methods: To answer this question, we probed at nanoscale lengths with FRET (Forster Resonance Energy Transfer), the interaction between A beta peptides and glycoprotein B (responsible of virus-cell binding) within the HSV-1 virion. Results: The experiments show an energy transfer between A beta peptides and glycoprotein B when membrane is intact. No energy transfer occurs after membrane disruption or treatment with blocking antibody. Conclusion: We concluded that A beta insert into viral membrane, close to glycoprotein B, and participate in virus neutralization.

Type de document:	Article
Mots-clés libres:	Alzheimer’s disease; FRET; HSV-1; amyloid-beta; glycoprotein B; interaction.
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	29 déc. 2023 03:32
Dernière modification:	29 déc. 2023 03:32
URI:	https://espace.inrs.ca/id/eprint/13351

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