Ramassamy, Charles 
ORCID: https://orcid.org/0000-0002-3252-5878; Haddad, Mohamed; Perrotte, Morgane; ben Khedher, Mohamed Raâfet; Madec, Elise et Fulop, Tamas
(2021).
Receptor for advanced glycation end products and glyoxalase-1 in the total circulating extracellular vesicles from mild cognitive impairment and Alzheimer's disease patients
In: Annual Meeting SFRR-E “Redox biology in the 21st century: a new scientific discipline, 2021, 15 - 18 June, Belgrade, Serbia.
Résumé
Background: Growing evidence supports that receptor for advanced glycation end products (RAGE) and glyoxalase-1 (GLO-1) are implicated in the pathophysiology of Alzheimer’s disease (AD). Extracellular vesicles (EVs) are nanovesicles secreted by almost all cell types, contribute to cellular communication, and are implicated in AD pathology. Recently, EVs are considered as promising tools to identify reliable biomarkers in AD. Objective: The aim of our study was to determine the levels of RAGE and GLO-1 in circulating EVs from MCI (mild cognitive impairment) and AD patients and to analyze their correlation with the clinical MMSE and MoCA scores. We have studied the possibility that neuronal cells could release and transfer GLO-1 through EVs. Methods: RAGE and GLO-1 levels were measured in circulating EVs by Luminex assay and Western blot. Released-EVs from SK-N-SH neuronal cells were isolated and GLO-1 levels were determined by Western bot. Results: Our data showed higher levels of RAGE in EVs from late AD patients while GLO-1 levels in EVs from early AD were lower as compared to control and MCI patients. Interestingly, levels of RAGE and GLO-1 in EVs were correlated with the cognitive scores regardless of age. For the first time, we demonstrated that GLO-1 was released from neuronal cells through EVs. Conclusion: Although more samples will be needed, our preliminary results highlighted the potential use of GLO-1 and RAGE levels in peripheral EVs as a clinical signature for AD progression. Keywords: alzheimer’s disease; mild cognitive impairment; extracellular vesicles; receptor for advanced glycation end products (RAGE); glyoxalase-1
| Type de document: | Document issu d'une conférence ou d'un atelier |
|---|---|
| Informations complémentaires: | Présentation orale Free Radical Biology and Medicine 177 (suppl 1): S17 |
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 30 juin 2022 14:05 |
| Dernière modification: | 30 juin 2022 14:05 |
| URI: | https://espace.inrs.ca/id/eprint/12426 |
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