Bruckner, Fernanda Prieto; Andrade, Patrícia Oliveira; de Souza Cascardo, Renan; Laliberté, Jean-François et Alfenas-Zerbini, Poliane (2022). Translationally controlled tumour protein: A protein necessary for potyvirus intracellular multiplication that supports plant infection by unrelated viruses Annals of Applied Biology , vol. 180 , nº 1. pp. 90-98. DOI: 10.1111/aab.12708.
Ce document n'est pas hébergé sur EspaceINRS.Résumé
The multifunctional protein translationally controlled tumour protein (TCTP) was previously identified as necessary for infection by the potyvirus pepper yellow mosaic virus. Using turnip mosaic virus (TuMV) as a model to study potyvirus biology, we confirmed that TCTP has a positive effect on virus infection. Living cell confocal microscopy demonstrated that TCTP colocalises with 6K2-tagged replication vesicles and with a perinuclear globular structure typically observed during potyvirus infection. Also, TCTP silenced protoplasts showed reduced virus accumulation, quantified by qRT-PCR, which suggests an effect on virus replication, translation or other intracellular process. Finally, TCTP silencing in plants reduced the accumulation of two species belonging to Orthotospovirus and a Begomovirus genus, which are not closely related to potyviruses. The results suggest that TCTP is a general susceptibility factor to several unrelated viruses. © 2021 Association of Applied Biologists.
| Type de document: | Article |
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| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 22 juin 2022 19:19 |
| Dernière modification: | 22 juin 2022 19:19 |
| URI: | https://espace.inrs.ca/id/eprint/12307 |
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