Yadav, Bhoomika; Yellapu, Sravan Kumar; Adjalle, Kokou D. 
ORCID: https://orcid.org/0000-0002-0836-6395; Drogui, Patrick ORCID: https://orcid.org/0000-0002-3802-2729 et Tyagi, Rajeshwar Dayal
(2021).
Comparative study on production and characterisation of extracellular polymeric substances (EPS) using activated sludge fortified with crude glycerol from different biodiesel companies.
Systems Microbiology and Biomanufacturing
, vol. 1
, nº 2.
pp. 208-222.
DOI: 10.1007/s43393-020-00017-5.
Résumé
Extracellular polymeric substances (EPS) are eco-friendly and economical biopolymers with a signifcant role in pollutant removal, bio-focculation, settling and dewatering of activated sludge. In this study, growth profle and EPS production by an EPS-producing microbial strain (isolated from wastewater sludge or WWS) was studied using activated sludge fortifed with glycerol. The study compared three crude glycerol samples as carbon sources from diferent biodiesel companies (BIO LIQ, BIOCARDEL, ROTHSAY) and their efect on EPS production and characterisation was observed. The maximum slime EPS (S-EPS) concentration (12.34 g/L) was produced when sludge fortifed with BIO-LIQ crude glycerol was used, higher than pure glycerol (10 g/L) at 72 h. The S-EPS was enhanced (16 g/L) when purifed BIO-LIQ glycerol (by acid treatment) was used. It was also observed that S-EPS concentration increased 1.6–2.6 times by using sludge fortifed with glycerol as compared to only glycerol (no sludge) as EPS production medium. In addition, the S-EPS produced from diferent glycerol samples yielded diferent characteristics in terms of protein-carbohydrate content, focculation activity and dewaterability. The S-EPS from purifed BIO-LIQ glycerol (with sludge) gave maximum focculation activity and dewaterability i.e. 93.71% and 60.2% respectively. The comparison between Ca(OH)₂ and NaOH used as alkaline sludge pre-treatment methods revealed that the EPS produced from Ca(OH)₂-treated sludge fortifed with glycerol solutions to be better in terms of protein content, focculation activity and dewaterability. Structural composition analysis using Fourier Transform Infrared Spectroscopy (FT-IR) revealed the presence of distinct functional groups (carboxyl, hydroxyl, amine groups) in the produced S-EPS.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | exopolysaccharides; bacterial polymers; slime; crude glycerol; activated sludge; impuritie |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 15 oct. 2021 18:16 |
| Dernière modification: | 15 févr. 2022 16:43 |
| URI: | https://espace.inrs.ca/id/eprint/11960 |
Gestion Actions (Identification requise)
 |
Modifier la notice |