Pinel, Laurie et Cyr, Daniel G. . Epididymal Basal Cells Expressing LGR5 Are Multipotent Adult Stem Cells In: Society for the Study of Reproduction-53rd SSR Annual Meeting, 8-12 July 2020, virtuel.
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The epididymal epithelium is pseudostratified and comprised of various cell types including principal, clear, narrow and basal cells. Basal cells share common properties of adult stem cells. They can differentiate into principal and clear cells in vivo, form organoids, self-renew, and differentiate in vitro . The characteristics of basal cells support the notion that these serve as a stem cell population residing at the base of the epididymal epithelium. However, there is currently no method to specifically identify epididymal stem cells. The present objective is to identify a specific marker of this population of stem cells in the epididymis. Leucine-rich repeat-containing G-protein coupled receptor 5 (LGR5) is a seven-transmembrane G-coupled receptor. It shares homology with two members of the LGR family, LGR4 and LGR6; all three proteins are receptors for R-Spondin growth factors, which modulate Wnt/Spondin signaling. LGR5 is a well-established marker of adult stem cells in a variety of tissues. We developed epididymal basal cell-derived organoids as a means to investigate epididymal stem cell differentiation. LGR5 was overexpressed in isolated basal cells of the rat epididymis and localized to the plasma membrane of basal cell-derived organoids. Its expression decreased as organoids differentiated. LGR5, 4 and 6 transcripts were expressed throughout the epididymis during postnatal development. LGR5 and LGR6 mRNA levels decrease during postnatal development while LGR4 mRNA levels remainedconstant. LGR5 was immunolocalized to undifferentiated columnar cells of the epididymis as early as PND7. However, as the epithelium differentiated, LGR5 became associated with basal cells. In the adult epididymis, LGR5 was localized primarily to basal cells, although weak staining was observed in narrow cells. Co-localization of LGR5 with the basal cell marker TP63 in the adult epididymis indicated the existence of at least 3 basal cell sub-types: LGR5 + /TP63 - , LGR5 + /TP63 + and LGR5 - /TP63 + . Together these data demonstrate that LGR5 is expressed in basal stem cells of the epididymis and that these cells have the ability to form organoids in vitro. Supported by CIHR, CIRD and the Canada Research Chairs Program.
| Type de document: | Document issu d'une conférence ou d'un atelier |
|---|---|
| Informations complémentaires: | Affiche scientifique numéro du résumé: 1961, pages 254-255 |
| Mots-clés libres: | - |
| Centre: | Centre INRS-Institut Armand Frappier |
| Date de dépôt: | 14 juill. 2021 15:55 |
| Dernière modification: | 14 juill. 2021 15:55 |
| URI: | https://espace.inrs.ca/id/eprint/11807 |
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