25-hydroxycholesterol impairs neuronal and muscular development in zebrafish

Jamadagni, Priyanka et Patten, Shunmoogum A. (2019). 25-hydroxycholesterol impairs neuronal and muscular development in zebrafish NeuroToxicology , vol. 75 . pp. 14-23. DOI: 10.1016/j.neuro.2019.08.007.

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Résumé

Oxysterols have essential effects on brain homeostasis and their levels are often altered in neurodegenerative and neuroinflammatory diseases. Several studies have demonstrated the cytotoxic effects of 25-HC on different cell lines, however, not much is known about its effects on neurons in vivo. In this study, we examined the effects of 25-HC exposure on the nervous system development in the zebrafish. We showed that survival rate of zebrafish embryos/larvae is significantly decreased at doses of 25-HC above 40 μM. 25-HC was found to affect the motility of zebrafish larvae, primary motor axon and muscle morphology. Furthermore, larvae treated with 25-HC showed a reduced neuronal network and number of HuC-positive cells in the brain. An increased cell death was also observed in both the brain and spinal cord of zebrafish treated with 25-HC. Interestingly, administration of 25-HC at later stages of development (24 and 48 h post fertilization) had no detrimental effects on motor axons. Altogether, our findings show that elevated levels of 25-HC may have important consequences on neuronal development and cell survival. © 2019

Type de document:	Article
Mots-clés libres:	25-hydroxycholesterol; Behavior; Neurodegeneration; Neurodevelopment Oxysterol Zebrafish
Centre:	Centre INRS-Institut Armand Frappier
Date de dépôt:	14 juill. 2021 16:16
Dernière modification:	14 juill. 2021 16:16
URI:	https://espace.inrs.ca/id/eprint/11521

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