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Psychrozymes as novel tools to biodegrade p-xylene and potential use for contaminated groundwater in the cold climate.

Miri, Saba, Davoodi, Seyyed Mohammadreza, Brar, Satinder Kaur, Rouissi, Tarek, Sheng, Yi et Martel, Richard ORCID: https://orcid.org/0000-0003-4219-5582 (2021). Psychrozymes as novel tools to biodegrade p-xylene and potential use for contaminated groundwater in the cold climate. Bioresource Technology , vol. 321 . p. 124464. DOI: 10.1016/j.biortech.2020.124464.

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Résumé

Sites contaminated by petroleum hydrocarbons in cold-climate regions have recently received significant attention due to their sensitive ecosystem and human health impacts. Two cold-adapted pseudomonas strains were isolated from contaminated groundwater and soil. As xylene monooxygenase from Pseudomonas synxantha S2TR-26 and catechol 2,3-dioxygenase from Pseudomonas mandelii S2TR-08, have a matching end product, they acted in symphony to degrade p-xylene. Their unique thermodynamic and kinetic behavior permits them to achieve rapid degradation of p-xylene at low temperatures (<15 °C). The results showed that the sequential action led to the conversion of 200 mg/l of p-xylene within 72 h and complete degradation after 120 h. The cocktail of these enzymes with a ratio of 1:1.5 (xylene monooxygenase: catechol 2, 3-dioxygenase) confirmed the complete degradation of p-xylene within 48 h at 15 °C. This approach will allow efficient biodegradation of p-xylene to minimize the bioremediation duration in cold-climate regions.

Type de document: Article
Mots-clés libres: biodegradation; cold-climate; p-xylene; monooxygenase; dioxygenase
Centre: Centre Eau Terre Environnement
Date de dépôt: 09 juin 2021 15:49
Dernière modification: 11 févr. 2022 14:49
URI: https://espace.inrs.ca/id/eprint/11498

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