Miri, Saba; Davoodi, Seyyed Mohammadreza; Brar, Satinder Kaur; Rouissi, Tarek; Sheng, Yi et Martel, Richard 
ORCID: https://orcid.org/0000-0003-4219-5582
(2021).
Psychrozymes as novel tools to biodegrade p-xylene and potential use for contaminated groundwater in the cold climate.
Bioresource Technology
, vol. 321
.
p. 124464.
DOI: 10.1016/j.biortech.2020.124464.
Résumé
Sites contaminated by petroleum hydrocarbons in cold-climate regions have recently received significant attention due to their sensitive ecosystem and human health impacts. Two cold-adapted pseudomonas strains were isolated from contaminated groundwater and soil. As xylene monooxygenase from Pseudomonas synxantha S2TR-26 and catechol 2,3-dioxygenase from Pseudomonas mandelii S2TR-08, have a matching end product, they acted in symphony to degrade p-xylene. Their unique thermodynamic and kinetic behavior permits them to achieve rapid degradation of p-xylene at low temperatures (<15 °C). The results showed that the sequential action led to the conversion of 200 mg/l of p-xylene within 72 h and complete degradation after 120 h. The cocktail of these enzymes with a ratio of 1:1.5 (xylene monooxygenase: catechol 2, 3-dioxygenase) confirmed the complete degradation of p-xylene within 48 h at 15 °C. This approach will allow efficient biodegradation of p-xylene to minimize the bioremediation duration in cold-climate regions.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | biodegradation; cold-climate; p-xylene; monooxygenase; dioxygenase |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 09 juin 2021 15:49 |
| Dernière modification: | 11 févr. 2022 14:49 |
| URI: | https://espace.inrs.ca/id/eprint/11498 |
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