Optimization of trace elements in purified glycerol for microbial lipid and citric acid production by Yarrowia lipolytica SKY7.

Kumar, Lalit; Yellapu, Sravan Kumar; Tyagi, Rajeshwar Dayal et Drogui, Patrick ORCID: https://orcid.org/0000-0002-3802-2729 (2021). Optimization of trace elements in purified glycerol for microbial lipid and citric acid production by Yarrowia lipolytica SKY7. Systems Microbiology and Biomanufacturing , vol. 1 , nº 1. pp. 76-89. DOI: 10.1007/s43393-020-00006-8.

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Résumé

Purified glycerol obtained after acid treatment of crude glycerol solution was used as the carbon source for lipid and citric acid production using Y. lipolytica SKY7. Although purified glycerol was high in phosphorus, it was important to investigate the impact of fortification of trace elements in the medium on cell growth, lipid and citric acid (CA) production. When all the trace elements (including phosphates and sulfates) required for growth and lipid production were added to the purified glycerol medium, high biomass (51.67 g/L) and lipid concentration (19.47 g/L) were observed at 96 h of fed-batch fermentation with low CA concentration of 5.42 g/L. The purified glycerol medium without additional trace elements gave low biomass (27.67 g/L), lipid concentration (9.35 g/L) at 80 h of fed-batch fermentation, but gave high CA concentration (24.51 g/L). When purified glycerol was provided with only sulfates or all elements except KH₂PO₄, low biomass (32.59 g/L and 38.52 g/L) and citric acid concentration (1 g/L and 2.42 g/L) were obtained at 96 h.

Type de document:	Article
Mots-clés libres:	Yarrowia lipolytica SKY7; purified glycerol; trace elements; microbial lipid; citric acid
Centre:	Centre Eau Terre Environnement
Date de dépôt:	03 févr. 2021 19:34
Dernière modification:	15 févr. 2022 16:40
URI:	https://espace.inrs.ca/id/eprint/11202

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