Elucidating the effect of impurities present in different crude glycerol sources on lipid and citric acid production by Yarrowia lipolytica SKY7.

Kumar, Lalit; Yellapu, Sravan Kumar; Yan, Song; Tyagi, Rajeshwar Dayal et Drogui, Patrick ORCID: https://orcid.org/0000-0002-3802-2729 (2020). Elucidating the effect of impurities present in different crude glycerol sources on lipid and citric acid production by Yarrowia lipolytica SKY7. Journal of Chemical Technology & Biotechnology , vol. 96 , nº 1. pp. 227-240. DOI: 10.1002/jctb.6531.

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Résumé

BACKGROUND

Crude glycerol is an industrial by‐product of biodiesel producing companies and requires high cost for purification. In fact, it is a good carbon source and can be used for lipid production from oleaginous microbes. However, crude glycerol has several impurities which may impact the cellular metabolism for lipid production.

RESULTS

In this study, crude glycerol from different sources was employed for lipid production and its impurities’ effect on the biomass and lipid production was investigated on Yarrowia lipolytica SKY7, which is a well‐known yeast for lipid and citric acid production. Growth inhibition was observed in BIOCARDEL, BIOLIQ and ROTHSAY glycerol when compared with pure glycerol. This was due to high sodium concentration in BIOCARDEL, high potassium concentration in BIOLIQ and high sulphur concentrations in ROTHSAY glycerol. Among three crude glycerol sources, the highest lipid concentration (14.78 g/L) was obtained using BIOCARDEL glycerol at 96 h. However, the higher citric acid concentration of 18.70 g/L in ROTHSAY glycerol and 12.00 g/L in BIOLIQ was obtained at 96 h when compared with 8.30 g/L in BIOCARDEL glycerol.

CONCLUSION

High potassium and sulphur concentration in glycerol medium inhibits cell growth and lipid production in Yarrowia lipolytica SKY7 while favors citric acid production.

Type de document:	Article
Mots-clés libres:	fermentation; lipids; biochemistry; biochemical engineering; biodiesel
Centre:	Centre Eau Terre Environnement
Date de dépôt:	24 juill. 2020 13:20
Dernière modification:	15 févr. 2022 16:32
URI:	https://espace.inrs.ca/id/eprint/10352

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