Purified crude glycerol by acid treatment allows to improve lipid productivity by Yarrowia lipolytica SKY7.

Kumar, Lalit; Yellapu, Sravan Kumar; Tyagi, Rajeshwar Dayal et Drogui, Patrick ORCID: https://orcid.org/0000-0002-3802-2729 (2020). Purified crude glycerol by acid treatment allows to improve lipid productivity by Yarrowia lipolytica SKY7. Process Biochemistry , vol. 96 . pp. 165-173. DOI: 10.1016/j.procbio.2020.06.010.

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Résumé

In this study, crude glycerol with high potassium concentration was purified using acid treatment and used as carbon source for lipid production using Yarrowia lipolytica SKY7. The crude glycerol was purified using phosphoric acid (pH 2) followed by centrifugation. When purified glycerol was used as carbon source for fermentation, higher biomass productivity (0.54 g/L/h) and lipid productivity (0.2 g/L/h) was observed at 96 h compared to crude glycerol. Results indicated that 6.32 g/L potassium in crude glycerol medium was inhibitory for cell growth and lipid production by Y. lipolytica. Yield coefficients, productivities and specific growth rates were calculated for each glycerol medium. The process performance with purified glycerol medium was comparable to that of pure glycerol medium. A higher lipid yield was obtained in purified glycerol medium (0.21 g/g glycerol) than crude glycerol medium (0.124 g/g glycerol). During purification of crude glycerol, KH2PO4 was also produced as by-product. This study provides a way for valorization of crude glycerol with high potassium concentration for microbial lipid production.

Type de document:	Article
Mots-clés libres:	Yarrowia lipolytica SKY7; glycerol purification; fed-batch fermentation; microbial lipid; citric acid
Centre:	Centre Eau Terre Environnement
Date de dépôt:	24 juill. 2020 13:26
Dernière modification:	15 févr. 2022 16:01
URI:	https://espace.inrs.ca/id/eprint/10345

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