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The central nervous system acts as a transducer of stress-induced masculinization through corticotropin-releasing hormone B.

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Castañeda Cortés, Diana C.; Padilla, Luisa F. Arias; Langlois, Valérie S.; Somoza, Gustavo M.; Fernandino, Juan I. (2019). The central nervous system acts as a transducer of stress-induced masculinization through corticotropin-releasing hormone B. Development , vol. 146 , nº 8. dev172866. DOI: 10.1242/dev.172866.

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Résumé

Exposure to environmental stressors, such as high temperature (HT), during early development of fish induces sex reversal of genotypic females. Nevertheless, the involvement of the brain in this process is not well clarified. In the present work, we investigated the mRNA levels of corticotropin-releasing hormone b (crhb) and its receptors (crhr1 and crhr2), and found that they were upregulated at HT during the crucial period of gonadal sex determination in medaka. In order to clarify their roles in sex reversal, biallelic mutants for crhr1 and crhr2 were produced by CRISPR/Cas9 technology. Remarkably, biallelic mutants of both loci (crhr1 and crhr2) did not undergo female-to-male sex reversal upon exposure to HT. Inhibition of this process in double corticotropin-releasing hormone receptor mutants could be successfully rescued through the administration of the downstream effector of the hypothalamic-pituitary-interrenal axis, cortisol. Taken together, these results reveal for the first time that the CNS acts as a transducer of masculinization induced by thermal stress.

Type de document: Article
Mots-clés libres: environmental stress; CRH; masculinization; CRISPR/Cas9; medaka
Centre: Centre Eau Terre Environnement
Date de dépôt: 29 nov. 2019 14:07
Dernière modification: 29 nov. 2019 14:07
URI: http://espace.inrs.ca/id/eprint/9551

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