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New immobilization method of anti-PepD monoclonal antibodies for the detection of Listeria monocytogenes p60 protein – Part A: Optimization of a crosslinked film support based on chitosan and cellulose nanocrystals (CNC)

Etty, Marie-Christine; D'Auria, Sabato; Shankar, Shiv; Salmieri, Stéphane; Couture, Julie; Baraketi, Amina; Jamshidan, Majid; Fraschini, Carole; Lacroix, Monique (2020). New immobilization method of anti-PepD monoclonal antibodies for the detection of Listeria monocytogenes p60 protein – Part A: Optimization of a crosslinked film support based on chitosan and cellulose nanocrystals (CNC) Reactive and Functional Polymers , vol. 146 , nº 104313. p. 1-10. DOI: 10.1016/j.reactfunctpolym.2019.06.021.

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Résumé

This paper presents the development of a support membrane based on chitosan, cellulose nanocrystals and glycerol (m-CCG) for the antibody immobilization by a covalent crosslinking using glutaraldehyde. The chemical characterization of the support by FTIR showed that m-CCG formation process was stabilized by the formation of hydrogen bonding between each component of m-CCG and the reactive amine groups allowing the antibody immobilization on m-CCG via glutaraldehyde. Moreover, this immobilization on m-CCG was optimized by mathematics modeling approaches, and it exhibited robustness and predictable detection in presence of 0.6% of cellulose nanocrystals (CNCs), 0.5 g of CCG solution per well, after 2 h of antibody immobilization. Results also showed that CNCs (0.6% w/v) was the most important factor of the optimization. At this concentration, CNCs improve the resistance of m-CCG during the crosslinking treatment by a modification of the surface topography and the reinforcement of the tensile strength of m-CCG at >30%.

Type de document: Article
Mots-clés libres: Antibody immobilization; Cellulose nanocrystals; Chitosan; Covalent crosslinking; Mathematical modeling; Cellulose; Cellulose derivatives; Crosslinking; Hydrogen bonds; Mathematical models; Monoclonal antibodies; Nanocrystals; Tensile strength; Topography; Cellulose nano-crystals; Cellulose nanocrystal (CNC)`; Cellulose nanocrystal (CNCs); Chemical characterization; Crosslinking treatment; Listeria monocytogenes; Chemical detection
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 03 févr. 2020 16:19
Dernière modification: 03 févr. 2020 16:22
URI: http://espace.inrs.ca/id/eprint/8561

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