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Differentiation by Immunodiffusion and by Quantitative Immunofluorescence Between 5-Fluorourachil-Treated and Normal Cells From a Toxinogenic Staphylococcus Aureus Strain

De Repentigny, Jacques; Sonea, Sorin; Frappier, Armand (1964). Differentiation by Immunodiffusion and by Quantitative Immunofluorescence Between 5-Fluorourachil-Treated and Normal Cells From a Toxinogenic Staphylococcus Aureus Strain Journal of bacteriology , vol. 88 , nº 2. p. 444-448.

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Résumé

Immunodiffusion and quantitative immunofluorescence can both detect antigenic changes produced 5-flourouracil (FU) in Staphylococcus aureus Wood 46 strain.When FU is added to the culture in their lgarithmie phase of growth, a number of bacterial antigens are no longer detectable by immunodiffusion and the itensity of the otal immunofluorescence of bacteria is diminished; thus, these antigens are either profoundly modified or no longer synthesized. Uracil and, less effectively, thymine can reverse the FU inhibitory effect on the synthesis of antigens, and the number of precipitin lines remains closer to controls. The immunochemical approach provides a new way of obtaining information on the action of thys pyrimidine analogue on metabolic processes in pathogenic bacteria. Microscopic quantitative immunofluorescence seems to be adaptable to give indirect information on chages in the metabolism or synthesis or antigens of a single bacterial cell.

Type de document: Article
Informations complémentaires: Recueil de tiré-à-part de la bibliothèque: A0285
Mots-clés libres: bacterial DNA, bacterial RNA, fluorouracil, uracil, article, chromatography, EXPERIMENTAL LAB STUDY, FLUORESCENT ANTIBODY, TECHNIC, fluorescent antibody technique, immunochemistry, pharmacology, research, serodiagnosis, Staphylococcus, DNA, BACTERIAL, PRECIPITIN TESTS, RNA, BACTERIAL
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 10 févr. 2020 02:09
Dernière modification: 10 févr. 2020 02:09
URI: http://espace.inrs.ca/id/eprint/8426

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