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Some biological properties of BCG after the extraction of lipids: the power to irritate tissues, the ability to prepare for and to evoke Bail's phenomenon, and the power to sensitize to tuberculin

Portelance, Vincent; Frappier, Armand (1958). Some biological properties of BCG after the extraction of lipids: the power to irritate tissues, the ability to prepare for and to evoke Bail's phenomenon, and the power to sensitize to tuberculin Canadian Journal of Microbiology , vol. 4 , nº 4. p. 409-420. DOI: 10.1139/m58-043.

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Résumé

Mycobacterium tuberculosis var. bovis BCG was treated with lipid solvents according to Macheboeuf's method and five progressively more fully extracted bacillary residues were obtained. Electron-microscope examination, together with the biological properties studied, showed that a major transformation was brought about in the bacillary residues by methanol extraction. The biological properties of the residues were studied quantitatively in guinea pigs. The power to irritate tissues (i.e., to produce tuberculous-like lesions in tissues), the ability to prepare for and to evoke Bail's phenomenon (i.e., reaction to the intraperitoneal introduction of bacillary bodies in sensitized guinea pig characterized by an immediate leucocytic response and death within a few hours) and the power to snsitize to tuberculine were directly proportional to the lipid contents of the bacillary bodies and decreased as the extraction progressed. The residues with the lower lipid contents were less irritant, less sensitizing, and less reactive. However, these properties never completely disappeared. The role of the lipids as related to the above properties of mycobacteria was thus indirectly established. With the doses and the routes of injection used, no obvious toxic effects in normal guinea pigs were observed.

Type de document: Article
Informations complémentaires: Recueil de tiré-à-part de la bibliothèque: A0147
Mots-clés libres: BCG vaccine; article; BCG vaccination
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 10 févr. 2020 16:14
Dernière modification: 10 févr. 2020 16:14
URI: http://espace.inrs.ca/id/eprint/8400

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