Rosabal-Rodriguez, Maikel; Mounicou, Sandra; Hare, Landis; Campbell, Peter G. C.
(2014).
How are trace elements managed in the cells of Chaoborus larvae to avoid ill-effects?
In: 35th Annual Meeting, Society of Environmental Toxicology and Chemistry - SETAC North America 2014, 9-13 novembre 2014, Vancouver, Canada.
Résumé
Larvae of the phantom midge Chaoborus have been proposed as sentinels to estimate the bioavailable concentrations of trace elements. Because they can tolerate acidic, metal-contaminated, environments, in which many other sentinels cannot live, they offer an opportunity to investigate the metal-handling strategies that allow them to prosper. Using Chaoborus larvae collected from lakes impacted by metals, we first determined the subcellular partitioning of Cd and Ni. The majority (up to 70%) of the Cd and Ni in larvae was detoxified by thermo-stable, cytosolic ligands. We then screened these metal-ligand complexes by SEC-ICP. The results revealed that cytosolic ligands with a similar molecular weight to metallothioneins (4-5 kDa) of the fruit fly (D. melangonaster) are important for Cd detoxification, whereas for Ni handling other ligands differing in their molecular weights (< 10 kDa) are involved.
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