Dépôt numérique
RECHERCHER

In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells

Téléchargements

Téléchargements par mois depuis la dernière année

Plus de statistiques...

Youssef, Samar Samir; Fahmy, Ahmed Mohamed; Omran, Moataza Hassan; Mohamed, Amr Saad; El Desouki, Mohamed Ali et El-Awady, Mostafa K. (2014). In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells BioMed Research International , vol. 2014 , nº 196712. pp. 1-7. DOI: 10.1155/2014/196712.

[thumbnail of In Vitro Inhibition of Hepatitis C Virus by Antisense Oligonucleotides in PBMC Compared to Hepatoma Cells.pdf]
Prévisualisation
PDF - Version acceptée
Disponible sous licence Creative Commons Attribution.

Télécharger (1MB) | Prévisualisation

Résumé

AIM: To assess the efficiency of phosphorothioate antisense oligodeoxynucleotide 1 (S-ODN1) on HCV translation inhibition in PBMC compared to hepatoma cells in vitro for the first time. MATERIALS AND METHODS: The study included 34 treatment naive HCV patients. IRES domain III and IV sequence variations were tested in 45 clones from 9 HCV patients. PBMC of HCV positive patients were subjected to S-ODN in vitro. Concomitantly HepG2 cells infected by the same patient's serum were also treated with S-ODN1 for 24 and 48 hours. Cellular RNA was tested for HCV plus and minus strands by reverse transcription polymerase chain reaction (RT-PCR). RESULTS: Sequence variations were seen in HCV IRES domain III only while domain IV was conserved among all the tested patient's clones. S-ODN1 successfully inhibited HCV translation in HepG2 cells, while in PBMC inhibition was partial. CONCLUSION: HCV IRES domain IV is more conserved than domain IIId in genotype 4 HCV patients. S-ODN against HCV IRES domain IV was not efficient to inhibit HCV translation in PBMC under the study conditions. Further studies testing other S-ODN targeting other HCV IRES domains in PBMC should be done.

Type de document: Article
Mots-clés libres: -
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 26 juin 2017 19:43
Dernière modification: 08 juin 2023 17:51
URI: https://espace.inrs.ca/id/eprint/3135

Gestion Actions (Identification requise)

Modifier la notice Modifier la notice