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Silver and fullerene nanoparticles' effect on interleukin-2-dependent proliferation of CD4 (+) T cells


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Côté-Maurais, Guillaume; Bernier, Jacques (2014). Silver and fullerene nanoparticles' effect on interleukin-2-dependent proliferation of CD4 (+) T cells Toxicology In Vitro , vol. 28 , nº 8. p. 1474-1481. DOI: 10.1016/j.tiv.2014.08.005.

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Immunotoxicity studies of nanoparticles on T cells addressed their effects on activation by T antigen receptor, but have neglected the regulation of proliferation by IL-2. In this study, the IL-2-dependent T lymphoblastoid WE17/10 cell line was used to compare silver (Ag-NPs) and fullerene (C60-NPs) nanoparticles' toxicity and evaluate whether these NPs could interfere with IL-2-dependent proliferation. Results have shown that Ag-NPs are more toxic, as they reduced cell viability at the highest concentration tested (100mug/ml), while C60-NPs have shown good biocompatibility. Characterization of NP suspensions by dynamic light scattering measured large aggregates for C60-NPs, whereas Ag-NPs were relatively stable and well dispersed. This translated into a much larger uptake of Ag-NPs compared to C60-NPs, as measured by flow cytometry. Proliferation measurements by CFSE following 72h incubation have shown that Ag-NPs decrease cell proliferation and C60-NPs slightly increase proliferation. CD25 expression was unchanged following exposure to C60-NPs, but was significantly increased by Ag-NPs' presence for short and long-term incubations. Analyses of three key signaling proteins activated by IL-2 receptor (Stat5, JNK and ERK1/2) by western immunoblotting have shown no effects from either NPs on Stat5 and JNK phosphorylation. ERK1/2 was slightly activated following a short exposure to Ag-NPs, while C60-NPs had no effect. Our results show that C60-NPs have good biocompatibility and do not interfere with IL-2-dependent proliferation. A deeper investigation would be needed for the case of Ag-NPs, since the mechanism of their action is still unclear.

Type de document: Article
Mots-clés libres: Fullerene; IL-2; Nanoparticle; Silver; T-lymphocyte; Toxicity
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 19 mai 2017 16:19
Dernière modification: 19 mai 2017 16:19
URI: http://espace.inrs.ca/id/eprint/3022

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