Dépôt numérique
RECHERCHER

One Aeromonas salmonicida subsp. salmonicida isolate with a pAsa5 variant bearing antibiotic resistance and a pRAS3 variant making a link with a swine pathogen

Massicotte, Marie-Ange; Vincent, Antony T.; Schneider, Anna; Paquet, Valérie E.; Frenette, Michel; Charette, Steve J. (2019). One Aeromonas salmonicida subsp. salmonicida isolate with a pAsa5 variant bearing antibiotic resistance and a pRAS3 variant making a link with a swine pathogen The Science of the Total Environment , vol. 690 . p. 313-320. DOI: 10.1016/j.scitotenv.2019.06.456.

Ce document n'est pas hébergé sur EspaceINRS.

Résumé

The Gram-negative bacterium Aeromonas salmonicida subsp. salmonicida is an aquatic pathogen which causes furunculosis to salmonids, especially in fish farms. The emergence of strains of this bacterium exhibiting antibiotic resistance is increasing, limiting the effectiveness of antibiotherapy as a treatment against this worldwide disease. In the present study, we discovered an isolate of A. salmonicida subsp. salmonicida that harbors two novel plasmids variants carrying antibiotic resistance genes. The use of long-read sequencing (PacBio) allowed us to fully characterize those variants, named pAsa5-3432 and pRAS3-3432, which both differ from their classic counterpart through their content in mobile genetic elements. The plasmid pAsa5-3432 carries a new multidrug region composed of multiple mobile genetic elements, including a Class 1 integron similar to an integrated element of Salmonella enterica. With this new region, probably acquired through plasmid recombination, pAsa5-3432 is the first reported plasmid of this bacterium that bears both an essential virulence factor (the type three secretion system) and multiple antibiotic resistance genes. As for pRAS3-3432, compared to the classic pRAS3, it carries a new mobile element that has only been identified in Chlamydia suis. Hence, with the identification of those two novel plasmids harboring mobile genetic elements that are normally encountered in other bacterial species, the present study puts emphasis on the important impact of mobile genetic elements in the genomic plasticity of A. salmonicida subsp. salmonicida and suggests that this aquatic bacterium could be an important reservoir of antibiotic resistance genes that can be exchanged with other bacteria, including human and animal pathogens.

Type de document: Article
Mots-clés libres: Aeromonas salmonicida subsp. salmonicida; Plasmid; pAsa5; Antibiotic resistance; Long-read sequencing; Gene transfer
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 11 janv. 2020 15:43
Dernière modification: 11 janv. 2020 15:43
URI: http://espace.inrs.ca/id/eprint/8595

Actions (Identification requise)

Modifier la notice Modifier la notice