Dépôt numérique
RECHERCHER

Extracellular DNA release, quorum sensing, and PrrF1/F2 small RNAs are key players in Pseudomonas aeruginosa tobramycin-enhanced biofilm formation

Téléchargements

Téléchargements par mois depuis la dernière année

Plus de statistiques...

Tahrioui, Ali; Duchesne, Rachel; Bouffartigues, Emeline; Rodrigues, Sophie; Maillot, Olivier; Tortuel, Damien; Hardouin, Julie; Taupin, Laure; Groleau, Marie-Christine; Dufour, Alain; Déziel, Éric; Brenner-Weiss, Gérard; Feuilloley, Marc G. J.; Orange, Nicole; Lesouhaitier, Olivier; Cornelis, Pierre; Chevalier, Sylvie (2019). Extracellular DNA release, quorum sensing, and PrrF1/F2 small RNAs are key players in Pseudomonas aeruginosa tobramycin-enhanced biofilm formation Npj Biofilms and Microbiomes , vol. 5 , nº 15. p. 1-11. DOI: 10.1038/s41522-019-0088-3.

[img]
Prévisualisation
PDF - Version publiée
Télécharger (3MB) | Prévisualisation

Résumé

Biofilms are structured microbial communities that are the leading cause of numerous chronic infections which are difficult to eradicate. Within the lungs of individuals with cystic fibrosis (CF), Pseudomonas aeruginosa causes persistent biofilm infection that is commonly treated with aminoglycoside antibiotics such as tobramycin. However, sublethal concentrations of this aminoglycoside were previously shown to increase biofilm formation by P. aeruginosa, but the underlying adaptive mechanisms still remain elusive. Herein, we combined confocal laser scanning microscope analyses, proteomics profiling, gene expression assays and phenotypic studies to unravel P. aeruginosa potential adaptive mechanisms in response to tobramycin exposure during biofilm growth. Under this condition, we show that the modified biofilm architecture is related at least in part to increased extracellular DNA (eDNA) release, most likely as a result of biofilm cell death. Furthermore, the activity of quorum sensing (QS) systems was increased, leading to higher production of QS signaling molecules. We also demonstrate upon tobramycin exposure an increase in expression of the PrrF small regulatory RNAs, as well as expression of iron uptake systems. Remarkably, biofilm biovolumes and eDNA relative abundances in pqs and prrF mutant strains decrease in the presence of tobramycin. Overall, our findings offer experimental evidences for a potential adaptive mechanism linking PrrF sRNAs, QS signaling, biofilm cell death, eDNA release, and tobramycin-enhanced biofilm formation in P. aeruginosa. These specific adaptive mechanisms should be considered to improve treatment strategies against P. aeruginosa biofilm establishment in CF patients' lungs.

Type de document: Article
Mots-clés libres: Biofilms, Antimicrobials, Pathogens
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 20 nov. 2019 21:34
Dernière modification: 20 nov. 2019 21:34
URI: http://espace.inrs.ca/id/eprint/8572

Actions (Identification requise)

Modifier la notice Modifier la notice