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Some biologic properties of myobacteria following the extraction of lipids; the power to initiate acquired resistance

Frappier, Armand; Portelance, Vincent; St-Pierre, Jacques (1959). Some biologic properties of myobacteria following the extraction of lipids; the power to initiate acquired resistance The American Review of Tuberculosis and Pulmonary Disease , vol. 79 , nº 3. p. 296-306. DOI: 10.1164/artpd.1959.79.3.296.

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Résumé

Saline suspensions of several bacillary residues of varying lipid contents were injected in guinea pigs to study their ability to stimulate active resistance. Gross tuberculosis was quantitatively estimated in the lungs, spleen, and liver of the sacrificed pigs. A new method of establishing numerical values corresponding as objectively as possible to the gravity of the experimental disease is described as well as the statistical assessment of the results with the chi-square test.

Massive and repeated doses of heat-killed BCG or of H37Rv residues prepared with a relatively rapid lipid extraction procedure afforded a significant degree of allergy and protection in guinea pigs. Massive doses of BCG residues submitted to a very prolonged process of lipid extraction conferred a low and irregular degree of allergy and retained only an insignificant power of protection. Relatively small doses of acetone-extracted BCG (residue I corresponding to a standard 60 mg. per ml. concentration of BCG) prepared by the same prolonged process of extraction seemed to possess an enhancing effect on the develpoment of experimental tuberculosis, although sensitizing no more than 22 per cent of the animals to tuberculin.

A filtrate of an old BCG culture, as well as a borate extract (mostly of protein nature) of mechanically disrupted BCg bacillary bodies, far from ummunizing the animals seemed to have enhanced their disease.

Type de document: Article
Informations complémentaires: Recueil de tiré-à-part de la bibliothèque: A0155
Mots-clés libres: lipid; article; Mycobacterium bovis; Mycobacterium tuberculosis; LIPIDS; MYCOBACTERIUM TUBERCULOSIS BOVIS
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 10 févr. 2020 16:07
Dernière modification: 10 févr. 2020 16:07
URI: http://espace.inrs.ca/id/eprint/8396

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