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Nouvelle technique pour l'étude in vivo de la sensibilité des staphylocoques aux substances antibactériennes; utilisation de l'embryon de poulet de 19 jours

Sonea, Sorin; Frappier, Armand (1955). Nouvelle technique pour l'étude in vivo de la sensibilité des staphylocoques aux substances antibactériennes; utilisation de l'embryon de poulet de 19 jours Canadian Journal of Microbiology , vol. 1 , nº 8. p. 597-604. DOI: 10.1139/m55-072.

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Résumé

The method of experimental staphylococcal infection using the 10-day-old chick embryo was adapted to the testing of antibacterial substances. This in vivo test consists in administering the antibacterial substance in the air sac of the egg, and in injecting the staphylococci into the embryo itself through the air sac.Contrary to other in vivo methods, lethal infections are easily obtained with most of the strains by injecting a very small number of staphylococci into untreated embryos: LD50 and MLD determination are obtained at will with all Staphylococcus strains; in this experimental infection, initial toxemia is avoided because of the effective inoculum is small and the red cells of the chick embryo are resistant to staphylococcal alpha-toxin. This method is an improvement over others previously described, which used rabbits or mice. The first phases of this infection could be compared in some respects with those of clinical cases. In the case of the in vivo sensitive strains, penicillin (50 units per egg) checked the infection when given at any time from the moment of inoculation up to six hours later. Two strains were penicillin resistant in vitro and in vivo but two other strains were resistant in vitro and susceptible in vivo to penicillin. Two different sulphomanides were effective against the chick-embryo infections obtained with most of the Staphylococcus strains used in this work.

Type de document: Article
Informations complémentaires: Recueil de tiré-à-part de la bibliothèque: A099
Mots-clés libres: MICROCOCCUS PYOGENES/effect of drugs on; PENICILLIN/effects; SULFONAMIDES/effects; antiinfective agent; penicillin; derivative; sulfanilamide; sulfanilamide derivative; sulfonamide; drug effects; in vitro study; Micrococcus; Staphylococcus; Staphylococcus aureus; Anti-Bacterial Agents; In Vitro Techniques; Penicillins; Sulfanilamides; Sulfonamides
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 10 févr. 2020 01:41
Dernière modification: 10 févr. 2020 01:41
URI: http://espace.inrs.ca/id/eprint/8385

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