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Recherches faites avec les mycobacteries marquees : VI- dispersion du bcg marque (au 32p) chez le cobaye

Sternberg, Joseph; Frappier, Armand (1956). Recherches faites avec les mycobacteries marquees : VI- dispersion du bcg marque (au 32p) chez le cobaye Annales de l'institut pasteur , vol. 90 , nº 5. p. 533-574.

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Résumé

The study of microbial disperson with the aid of labelled microbes must be done by superimpozing a biological control (microbia numeration) upon the results offered by the radioactivity assay. The ratio 32P/microbe has been named microbial specific activity.

In normal guinea pig injected with 10S microbes, almost half of the injected dosis has been found at the inoculation site, whereas the microbial mass recuperated in all the other tissues together aerages at most 1% of the total injected. The number of living microbes found at the inoculation site of the vaccinated in the other examined tissues (liver, spleen, lungs) the number of microbes is considerably diminished in vaccinated (100 to 500 times) as compared to the controls.

The radioactivity assay of the tissues gave different results than that of the direct microbial count, namely a considerable increase of the tissular concentration of the isotope, which averages nearly 50% of the injected dosis, insted of the 1% of the tissular microbes as found by the bacteriological procedure.

The vaccination with BCG does not seem to alter the pattern of distribution of the isotope in the tissues, but the microbial specific activity is considerably increased in vaccinated animals, because of the diminution of the living microbial units in the examined tissues.

In animals injected with heat-killed labelled BCG, the pattern of distribution of 32P differs from that in animals injected with living microbes, especially in the skin, which concentrates more 32P in the former case. This is even more marked in vaccinated animals.

The pattern of lissular distribution of 32P in experiments with labelled microbes is different from the distribution schema of the mineral radiophosphate. In animals injected simultaneously with 32PO4Na2H and living non labelled BCG, the highest percentage of the isotope (38%)was excreted in the urine, and almost an equal part was fixed in the bones; the concentration at the inoculation site is far less important than in the former experiments.

There is an acceleration of the phosphate turnover in vaccinated animals, noted in experiments with labelled microbes as well as in the experiments with mineral radiophosphate.

Type de document: Article
Mots-clés libres: 09-0244849; bcg; cobaye; dispersion; marque; marquee; mycobacterie
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 07 févr. 2020 15:43
Dernière modification: 07 févr. 2020 15:43
URI: http://espace.inrs.ca/id/eprint/8308

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