Yellapu, Sravan Kumar; Klai, Nouha; Kaur, Rajwinder; Tyagi, Rajeshwar Dayal et Surampalli, Rao Y. (2019). Oleaginous yeast biomass flocculation using bioflocculant produced in wastewater sludge and transesterification using petroleum diesel as a co-solvent. Renewable Energy , vol. 131 . pp. 217-228. DOI: 10.1016/j.renene.2018.06.066.
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In this research a new process of harvesting the oleaginous yeast biomass (Yarrowia lipolytica) by flocculation followed by cell wall disruption and lipid extraction with petroleum diesel as a solvent was developed. Alum and calcium chloride along with the extracellular polymeric substance (EPS) as a flocculant were evaluated for lipid bearing biomass settling. The maximum flocculation activity of biomass using calcium chloride (36 mM) in combination with EPS (5.85 mg EPS/g biomass) or Alum (1.2 mM) with EPS (18 mg EPS/g biomass) was 74.3 and 79% and the settling velocity was 2.93 and 1.46 mm/s, respectively. To have a similar efficiency of biomass settling, 3.07 times less dosage of EPS was required in combination with calcium chloride than required with Alum. Further, settled biomass (166 g/L) was treated with N-lauroyl sarcosine (N-LS) to disrupt the cellular structure and release lipid. The released lipid was separated from cell debris and water using petroleum diesel (co-solvent) and maximum lipid recovery efficiency of 94.7 ± 1.2 % (w/w) was observed.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | oleaginous yeast; flocculation; extracellular polymeric substance; ex-situ transesterification; petroleum diesel |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 23 oct. 2018 20:17 |
| Dernière modification: | 23 oct. 2018 20:17 |
| URI: | https://espace.inrs.ca/id/eprint/7747 |
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