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The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology

Stodola, Jenny Karen; Dubois, Guillaume; Le Coupanec, Alain; Desforges, Marc; Talbot, Pierre J. (2018). The OC43 human coronavirus envelope protein is critical for infectious virus production and propagation in neuronal cells and is a determinant of neurovirulence and CNS pathology Virology , vol. 515 . p. 134-149. DOI: 10.1016/j.virol.2017.12.023.

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Résumé

The OC43 strain of human coronavirus (HCoV-OC43) is an ubiquitous respiratory tract pathogen possessing neurotropic capacities. Coronavirus structural envelope (E) protein possesses specific motifs involved in protein-protein interaction or in homo-oligomeric ion channel formation, which are known to play various roles including in virion morphology/assembly and in cell response to infection and/or virulence. Making use of recombinant viruses either devoid of the E protein or harboring mutations either in putative transmembrane domain or PDZ-binding motif, we demonstrated that a fully functional HCoV-OC43 E protein is first needed for optimal production of recombinant infectious viruses. Furthermore, HCoV-OC43 infection of human epithelial and neuronal cell lines, of mixed murine primary cultures from the central nervous system and of mouse central nervous system showed that the E protein is critical for efficient and optimal virus replication and propagation, and thereby for neurovirulence. © 2017 Elsevier Inc.

Type de document: Article
Mots-clés libres: Coronavirus; E protein; HCoV-OC43; Pathogenesis; PDZ binding motif; Transmembrane domain; Virus production
Centre: Centre INRS-Institut Armand Frappier
Date de dépôt: 22 mars 2019 14:32
Dernière modification: 22 mars 2019 14:32
URI: http://espace.inrs.ca/id/eprint/6750

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