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Valorization of crude glycerol and eggshell biowaste as media components for hydrogen production: A scale-up study using co-culture system.

Pachapur, Vinayak Laxman; Das, Ratul Kumar; Brar, Satinder Kaur; Le Bihan, Yann; Buelna, Gerardo (2017). Valorization of crude glycerol and eggshell biowaste as media components for hydrogen production: A scale-up study using co-culture system. Bioresource Technology , vol. 225 . p. 386-394. DOI: 10.1016/j.biortech.2016.11.114.

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Résumé

The properties of eggshells (EGS) as neutralizing and immobilizing agent were investigated for hydrogen (H₂) production using crude glycerol (CG) by co-culture system. Eggshells of different sizes and concentrations were used during batch and repeated-batch fermentation. For batch and repeated-batch fermentation, the maximum H₂ production (36.53 ± 0.53 and 41.16 ± 0.95 mmol/L, respectively) was obtained with the EGS size of 33 μm < x₅ < 75 μm. Hydrogen production increased with the decreasing size of EGS. Eggshells maintained the fermentation pH (6.00–6.30) and provided immobilization support as confirmed by scanning electron microscopy. As media components, the EGS concentration of 0.25% (w/v) was found to be optimum for maximum H₂ production (31.66 ± 0.55 mmol/L) and the production profile was comparable to H₂ production (32.07 ± 0.92 mmol/L) obtained with all media components. In scale-up study with semi-continuous bioreactor (7.5 L), almost 1.5-fold increase (in comparison to mono-culture) i.e. 312.12 mmol-H₂/L-of medium with 86.65% glycerol utilization was obtained.

Type de document:
Mots-clés libres: crude glycerol; co-culture; eggshell; hydrogen; immobilization; neutralization
Centre: Centre Eau Terre Environnement
Date de dépôt: 16 juin 2017 19:09
Dernière modification: 16 juin 2017 19:09
URI: http://espace.inrs.ca/id/eprint/5174

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