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Kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7.

Kuttiraja, Mathiazhakan; Dhouha, Ayed; Tyagi, Rajeshwar Dayal (2016). Kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7. Bioresource Technology , vol. 221 . p. 234-240. DOI: 10.1016/j.biortech.2016.09.015.

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Résumé

The objective of this work was to study the kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7. The model terms glycerol concentration inoculum and C/N ratio with inoculum were found to be significant for lipid production. Lipid production was found to be higher in glycerol 82.5 g/L, C/N ratio 75 and inoculum volume 6.25%. Optimized culture conditions were tested at 15 L bench scale reactor. The biomass concentration and lipid content obtained was 29.5 g/L and 50% (w/w), respectively. The yield coefficients were calculated and found to be 0.332 g/g (g biomass/g of glycerol) of biomass and 0.179 g/g (g lipid/g glycerol consumed) for lipid. Observed rates of lipid production show lipid production from 30 h of fermentation. Out of the total glycerol consumed, 41.1% glycerol was converted into biomass, lipid, and citric acid.

Type de document:
Mots-clés libres: microbial lipids; Yarrowia lipolytica; fermentation; RSM optimization; biomass
Centre: Centre Eau Terre Environnement
Date de dépôt: 29 nov. 2016 21:25
Dernière modification: 29 juin 2017 13:31
URI: http://espace.inrs.ca/id/eprint/4743

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