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Evaluation of inhibitory measures for food spoiler yeast candida krusei during fermentation process by chemical, biochemical and nanoparticle approaches.

Bhattacharya, Indrani; Bezawada, Jyothi; Yadav, Jay Shankar Singh; Yan, Song; Tyagi, Rajeshwar Dayal; Surampalli, Rao Y. (2016). Evaluation of inhibitory measures for food spoiler yeast candida krusei during fermentation process by chemical, biochemical and nanoparticle approaches. Journal of Microbiology, Biotechnology and Food Sciences , vol. 5 , nº 6. p. 509-517. DOI: 10.15414/jmbfs.2016.5.6.509-517.

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Résumé

Screening of chemical, biochemical and biomolecule-nanoparticle methods for the inhibition of Candida krusei were evaluated without hampering the growth of dairy yeast Kluyveromyces marxianus. The effective inhibition was observed with the help of H₂O₂, Williopsis saturnus, at specific combination of pH and temperature (pH 5.0 and 40 °C) and Ag-KT4561 nanoparticles among the various methods used. However, the most efficient inhibition was observed with Ag-KT4561 nanoparticles. In general H₂O₂ works best at pH range 4.0 to 10.0 and at temperature 30 °C or above. H₂O₂ concentration of 4000 ppm at 45 °C and pH 5.5 exhibited significant inhibition of C. krusei, while K. marxianus remains unaffected. But, when used with lyophilized supernatant of W. saturnus, 2400 ppm H₂O₂ was effective. Further, nanoparticle with silver was synthesized to reduce the quantity of killer protein and enhance the efficiency of protein. Complete inhibition of C. krusei was observed at 350 µM of synthesized silver nano-particle (AgNPs) of the killer protein from W. saturnus, with little effect on K. marxianus concentration. A stability test confirms the effect of protein silver nanoparticles on C. krusei for more than 20 weeks without any change in pH and temperature. Thus, the nanoparticles could be potentially used for inhibition of C. krusei without affecting the growth of K. marxianus and the process could be run non-aseptically.

Type de document: Article
Mots-clés libres: H₂O₂; spoiler yeast; inhibition; killer protein; Ag-KT4561 bio-molecule nanoparticles; green chemistry
Centre: Centre Eau Terre Environnement
Date de dépôt: 03 mai 2018 15:30
Dernière modification: 03 mai 2018 15:30
URI: http://espace.inrs.ca/id/eprint/4684

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