Rosabal, Maikel; Mounicou, Sandra; Hare, Landis et Campbell, Peter G. C. (2016). Metal (Ag, Cd, Cu, Ni, Tl, and Zn) Binding to Cytosolic Biomolecules in Field-Collected Larvae of the Insect Chaoborus. Environmental Science & Technology , vol. 50 , nº 6. pp. 3247-3255. DOI: 10.1021/acs.est.5b05961.
Ce document n'est pas hébergé sur EspaceINRS.Résumé
We characterized the biomolecules involved in handling cytosolic metals in larvae of the phantom midge (Chaoborus) collected from five mining-impacted lakes by determining the distribution of Ag, Cd, Cu, Ni, Tl, and Zn among pools of various molecular weights (HMW: high molecular weight, >670?40 kDa; MMW: medium molecular weight, 40? <1.3 kDa; LMW: low molecular weight, <1.3 kDa). Appreciable concentrations of nonessential metals were found in the potentially metal-sensitive HMW (Ag and Ni) and LMW (Tl) pools, whereas the MMW pool, which includes metallothioneins (MTs) and metallothionein-like proteins and peptides (MTLPs), appears to be involved in Ag and Cd detoxification. Higher-resolution fractionation of the heat-stable protein (HSP) fraction revealed further differences in the partitioning of nonessential metals (i.e., Ag = Cd ? Ni ? Tl). These results provide unprecedented details about the metal-handling strategies employed by a metal-tolerant, freshwater animal in a field situation.
| Type de document: | Article |
|---|---|
| Mots-clés libres: | Metals; biomolecules; freshwater larvae; chaoborus; biomonitoring; |
| Centre: | Centre Eau Terre Environnement |
| Date de dépôt: | 20 déc. 2016 16:42 |
| Dernière modification: | 12 nov. 2018 16:44 |
| URI: | https://espace.inrs.ca/id/eprint/4139 |
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